Question

during coiling or uncoiling or mapping of DNA what would be the change in entropy​

Answer 1

Answer:

Rabbit skeletal myosin was prepared by the method of Godfrey and Harrington (22) and digested with α-chymotrypsin to myosin rod. LMM was prepared by α-chymotryptic digestion of the myosin rod and separation by centrifugation at low ionic strength. Myosin rod and LMM were affinity purified to remove all myosin heads by ultracentrifugation in the presence of excess F-actin at high ionic strength. S2 was prepared by α-chymotryptic digestion of the myosin rod, ethanol precipitation, and ion exchange fast protein liquid chromatography (FPLC) on DEAE sepharose (23,24). Single-headed myosin was prepared from myosin by limited α-chymotryptic digestion, centrifugation, and ion exchange FPLC on DEAE sepharose as previously described (4). Immediately before force microscopy experiments, the myosin and its subfragments were sized by gel filtration chromatography with Superose 6 in high ionic strength buffer (0.5 M KCl, 20 mM imidazole, 1 mM TCEP, pH 7.0) to ensure a monodisperse preparation. Purity and approximate molecular masses were assessed by SDS-PAGE and densitometry.