During electrophoresis denaturation of double stranded dna is brought about by application of current?
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Electrophoresis is useful in the separation of proteins of different structures. To enable the separation any protein should be brought to its monomeric state initially.for this purpose SDS is used to denature the proteins. The disulphide bonds present in the protreins are broken down by beta-mercapto ethanol which is used in the reducing dye to reduce the bonds between them
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