Question

during the biuret test, what is the reason for using 40% NaOH with filtrate obtained after half and full saturation with ammonium sulfate​

Answer 1

Answer:

Salt Saturation Test is a test for the precipitation of proteins and their differentiation from other proteins by using the salting-out technique. Salting out is the process of precipitation of proteins by increasing the concentration of salt in the solution. The salts used in this technique are mostly neutral mineral salts like MgSO4, Na2SO4, and (NH)2SO4. The salt solubilization test also helps in the differentiation of proteins as some proteins precipitate under lower salt concentrations while others require higher concentrations. The reagent used for the test might differ depending on the preference with salts like ammonium sulfate being used due to their increased solubility. The salt saturation test can be performed either as half saturation or full saturation dependin