during the performance of the simple staining procedure, you failed to heat your E.coli smear preparation. upon microscopic examination, how would you expect this slide to differ from the correctly prepared slide.???
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Before bacteria can be stained, a smear of bacteria must be made on a slide and heat fixed. ... Heat fixing denatures bacterial enzymes, preventing them from digesting cell parts, which causes the cell to break, a process called autolysis. The heat also enhances the adherence of bacterial cells to the slide.
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