Question

During transcription, RNA polymerase holoenzyme binds to a gene promoter and assumes a saddle-like structure. What is it's DNA-binding sequence ?

Answer 1

Binding site sequence that is responsible for the saddle shape of DNA during transcription is TATA box or Goldberg-Hogness box.

1. It is a non coding region in the promoter of a gene.
2. It is situated approximately 24 base pairs upstream to the transcription start point.
3. This contains 'AT rich' nucleotide sequence of 7-8  base pair length.
4. This acts as the binding site for pre-initiative complex formation for the transcription.