during triglyceride formation, three water molecules are released. "
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You don't clearly defined your question, but i provide something about triglyceride formation.
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In this process 3 molecules of fatty acid (stearic acid) combine with 1 molecule of glycerol, with the removal of 3 molecules of water (blue). Both glycerol and carboxylic acids have -OH bonds, which together contribute 2 hydrogen atoms and 1 oxygen atom to form the water. Three ester linkages (green) are formed as a result. The resulting compound (glyceryl tristearate ) is also called a triglyceride, or a neutral fat.
Glycerol is a trihydric alcohol, having 3 -OH groups.
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