Question

Ecori is a restriction enzyme which cuts dna molecule between g and a bases only when the sequence is

Answer 1

EcoRI digestion produces "sticky" ends, whereas SmaI restriction enzyme cleavage produces "blunt" ends: Recognition sequences in DNA differ for each restriction enzyme, producing differences in the length, sequence and strand orientation (5' end or 3' end) of a sticky-end "overhang" of an enzyme restriction.

Answer 2

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➡️Like all enzymes, a restriction enzyme works by shape-to-shape matching.

➡️When it comes into contact with a DNA sequence with a shape that matches a part of the enzyme, called the recognition site, it wraps around the DNA and causes a break in both strands of the DNA molecule.

➡️Restriction enzymes are DNA-cutting enzymes found in bacteria (and harvested from them for use).

➡️Because they cut within the molecule, they are often called restriction endonucleases.

➡️In order to be able to sequence DNA, it is first necessary to cut it into smaller fragments.

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