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Edit Answer Submit Ans Question Description: Q2: In a recombinant protein expression process the host microorganism. Picha pastoris needs to be separated from the culture supernatant after production A pilot scale bioreactor with a working volume of 250 liters is used for this process and the final biomass concentration is 330 g/L. Considering the separation should be finished in 1 hour a) Calculate the sigma factor () of a continuous disc stack centrifuge that can fulfil this task assuming density of the microorganism is 1.2 g/L, average size is 3jim (round shape), density of the supernatant is 1.05 g/L. b) If you wanted to reduce the duration of this separation process by 50%.. what would you change? Please consider and mention the benefits and disadvantages of such a change. If the protein produced in Q2 is a single chain variable fragment of an antibody containing a 6x Histidine tag a) What kind of chromatography would you use for purification of this protein? Briefly explain this specific method b) Assuming product yield per biomass (YK) is 0.0006 g protein/g biomass, calculate the volume of chromatography resin material required for one batch purification. Do not forget to find out the max protein binding capacity of the chromatography resin​

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14. Jerry moves 4 km from A to B and 1 poin 5 km towards C as shown in the figure. Total distance travelled by him is 9 km. Find out the displacement from his initial point A? (Note: use Pythagoras theorem) D 4 km al B c (a) 7.40 km (b) 6 km (c) 5 km O (d) 6.40 k