Biology, asked by IpshitaBasu6799, 1 year ago

Effect of consortia on volatile acid production during anaerobic digestion

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Answered by vanshita7760
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Bioaugmentation with a mixture of microorganisms (Bacteria and Archaea) was applied to improve the anaerobic digestion of sewage sludge. The study was performed in reactors operating at a temperature of 35 °C in semi-flow mode. Three runs with different doses of bioaugmenting mixture were conducted. Bioaugmentation of sewage sludge improved fermentation and allowed satisfactory biogas/methane yields and a biodegradation efficiency of more than 46%, despite the decrease in hydraulic retention time (HRT) from 20 d to 16.7 d. Moreover, in terms of biogas production, the rate constant k increased from 0.071 h−1 to 0.087 h−1 as doses of the bioaugmenting mixture were increased, as compared to values of 0.066 h−1 and 0.069 h−1 obtained with sewage sludge alone.


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Answered by Ritiksuglan
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Hey mates your answer is here

.Bioaugmentation with a mixture of microorganisms (Bacteria and Archaea) was applied to improve the anaerobic digestion of sewage sludge. The study was performed in reactors operating at a temperature of 35 °C in semi-flow mode. Three runs with different doses of bioaugmenting mixture were conducted. Bioaugmentation of sewage sludge improved fermentation and allowed satisfactory biogas/methane yields and a biodegradation efficiency of more than 46%, despite the decrease in hydraulic retention time (HRT) from 20 d to 16.7 d. Moreover, in terms of biogas production, the rate constant k increased from 0.071 h−1 to 0.087 h−1 as doses of the bioaugmenting mixture were increased, as compared to values of 0.066 h−1 and 0.069 h−1 obtained with sewage sludge alone. Next-generation sequencing revealed that Cytophaga sp. predominated among Bacteria in digesters and that the hydrogenotrophic methanogen Methanoculleus sp. was the most abundant genus among Archaea.

Keywords: anaerobic digestion, wastewater sludge, bioaugmentation, metagenome, NGS

1. Introduction

The development of wastewater treatment technology, together with the implementation of environmental legislation, has successfully protected the aquatic environment from pollution. However, at the same time, sewage sludge is generated as the by-product of the wastewater treatment. Sewage sludge is becoming a worldwide environmental problem because of its increasing production and its high contents of organic matter, pathogens, and heavy metals.

Anaerobic digestion is a biological process that can degrade organic material by the concerted action of a wide range of microorganisms in the absence of oxygen. However, the advantages of the anaerobic digestion process in the treatment of sewage are still far from being optimized. Regardless of the temperature conditions, only around 50% to 60% of the organic matter can be degraded, leaving a large potential of increasing the biogas production [1]. A better understanding of the basic mechanisms occurring in the digester, conducting the process at high temperatures, application of different kinds of pre-treatment methods (freezing/thawing; cavitation), phase separation, and, recently, bioaugmentation has been applied to improve the anaerobic digestion.

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