Effects of fluoride on autophagy level in human neuroblastoma sh-sy5y cells
Answers
OBJECTIVE: To explore the effects of fluoride on autophagy level in human neuroblastoma SH-SY5Y cells.
METHODS: SH-SY5Y cells were treated with different concentrations( 20, 40, 60 mg/L) of fluoride for 24 h. Transmission electron microscope( TEM) was used to detect the ultrastructure of autophagosomes in SH-SY5Y cells. Acridine orange staining was used to examine the form of autophagic vesicles and Western blotting was used to check the expression levels of autophagy relevant proteins of Atg5, LC3 and P62 in SH-SY5 Y cells.
RESULTS: The TEM results showed the presence of autophagic ultrastructure in SH-SY5Y cells and fluoride could decrease the amount of autophagosomes. Acridine orange staining results showed that the amount of autophagic vesicles were obviously decreased in 40 and 60 mg/L Na F-treated groups compared with control, and 60 mg/L Na F-treated group was decreased the most. The expression level of Atg5 and LC3-II protein was dose-dependently decrease and P62 protein was dosedependently increased compared with control( P < 0. 05).
CONCLUSION: Fluoride maysuppress the level of autophagy in SH-SY5Y
IF YOU LIKE PLEASE MARK AS BRAINLIEST