Biology, asked by tdprajapati7746, 1 year ago

Ehat is the importance of treating bacterial cell with divalent cation during r dna transformation

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Answered by visroliyadevesh

The heat shock step strongly depolarizes the cell membrane of CaCl2-treated cells. Thus, the decrease in membrane potential lowers the negativity of the cell's inside potential which ultimately allows the movement of negatively charged DNA into the cell's interior.

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