Emasculation diagrams and comment.exprement
Answers
Procedure
(i) Select a flower in bud condition where antheses has not occurred. Open
the bud carefully and remove the stamens ( Mark this as
female parent plant.
(ii) Cover the emasculated flower with a plastic
bag to protect it from undesired pollen
(Bagging) (Fig. 12.2). The bag should be
held securely in place with a paper clip/
string/thread. Select the size of bag in
accordance with the flower size. Bags
must be transparent with minute pores.
(iii) Bring into physical contact anthers of a
desired male plant containing mature
pollen grains with the stigmatic surface
of emasculated female flower (Fig. 12.3).
Use tweezers/brush if necessary to dust
the stigmatic surface with pollen.
(iv) Cover the pollinated flower again with the
bag immediately. For identification, label
the female parent (Tagging). Each
pollinated flower should bear a label
containing the name of the seed parent,
the letter X (to signify a cross), the name
of the pollen parent, and the date on which
the cross was effected
