Enzymology of recombinant dna technology by biology discussion
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The following points highlight the seven main stages of recombinant DNA technology. The stages are: 1. Isolation of the Genetic Material (DNA) 2. Cutting of DNA at Specific Locations 3. Isolation of Desired DNA Fragment 4. Amplification of Gene of Interest using PCR 5. Ligation of DNA Fragment into a Vector 6.Insertion of Recombinant DNA into the Host Cell/Organisms 7. Obtaining or Culturing the Foreign Gene Product
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