Estimate the various sterilisation techniques used in tissue culture work
Answers
The basic techniques of some important tissue cultures are:
(1) Meristem Culture (2) Embryo Culture (3) Anther Culture and Haploid Production (4) Protoplast Culture and Somatic Hybridization (5) Ovule Culture and (6)Micro Propagation.
Technique # 1. Meristem Culture:
(i) One can use an explant that contains pre-existing shoot meristems and produce shoots from them. Such cultures are known as meristem culture.
It is used in:
(i) Production of virus-free plants.
(ii) Germplasm conservation,
(iii) Production of transgenic plants.
(iv) Rapid clonal multiplication.
Basic Techniques of Some Important Tissue Cultures | Biotechnology
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The basic techniques of some important tissue cultures are:
(1) Meristem Culture (2) Embryo Culture (3) Anther Culture and Haploid Production (4) Protoplast Culture and Somatic Hybridization (5) Ovule Culture and (6)Micro Propagation.
Technique # 1. Meristem Culture:
(i) One can use an explant that contains pre-existing shoot meristems and produce shoots from them. Such cultures are known as meristem culture.
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(ii) The explants commonly used in meristem culture are shoot tips and nodal segments.
(iii) These explants are cultured on a medium containing a cytokine (generally BAP).
(iv) The plantlets thus obtained are subjected to hardening and ultimately established in the field.
(v) Meristem culture is carried out in potato, banana, cardamom, orchids (proto-corm stage), sugarcane, strawberry, sweet potato, etc.
It is used in:
(i) Production of virus-free plants.
(ii) Germplasm conservation,
(iii) Production of transgenic plants.
(iv) Rapid clonal multiplication.
Technique # 2. Embryo Culture:
(i) Embryo culture is the technique of taking out young embryos from developing seeds and their growth on culture medium to form seedlings and then young plants.
(ii) Older embryos are more easily cultured because they are almost completely self sufficient so that they require a simple inorganic medium with a source of energy.
(iii) The immature embryos additionally require high osmotic pressure, higher potassium level, abscise acid, ammonium nitrogen and reduced oxygen tension.
Answer:
Tissue culture. Tissue culture, a method of biological research in which fragments of tissue from an animal or plant are transferred to an artificial environment in which they can continue to survive and function. The cultured tissue may consist of a single cell, a population of cells, or a whole or part of an organ.
Explanation:
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