Question

exemplify the role of Mass ( denisty ) centrifugation.
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Answer 1

Explanation:

Suitable Density Gradient Medium Selection. The primary function of density gradient centrifugation is to separate particles, either on the basis of their buoyancy density or their rate of sedimentation.

Density gradient centrifugation is the separation of molecules where the separation is based on the density of the molecules as they pass through a density gradient under a centrifugal force.

Principle of Density gradient centrifugation

Density gradient centrifugation is based on the principle that molecules settle down under a centrifugal force until they reach a medium with the density the same as theirs.

In this case, a medium with a density gradient is employed, which either has to decrease density or increasing density.

Molecules in a sample move through the medium as the sample is rotated creating a centrifugal force.

The more dense molecules begin to move towards the bottom as they move through the density gradient.

The molecules then become suspended at a point in which the density of the particles equals the surrounding medium.

In this way, molecules with different densities are separated at different layers which can then be recovered by various processes.

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