Question

Exercise:
Q no. 1: When establishing a density gradient in a test tube, is it better to build from the heaviest
layer up or the lightest layer down in centrifugation? Why?
Ans:
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Answer 1

Answer:

The sample is introduced at the top of the gradient and allowed to spin for a certain period of time during which the particles migrate at different rates toward the bottom of the tube. In an isopycni...

Answer 2

Answer:

• You're probably familiar with a centrifuge, a machine that spins a sample in a suitable holder.
• Particles in the sample holder settle out because the centrifugal force produced by the spinning is stronger than the force of gravity.
• You might employ a technique called density gradient centrifugation.
• When utilizing ultracentrifugation to purify chemicals, this is the preferable method.
• The tube's bottom layer is the heaviest or densest, and its top layer is the lightest.
• After that, the sample is positioned on top of the gradient and spun in the centrifuge.
• Sucrose or cesium iodide are the materials that are most frequently employed to create density gradients.

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