EXERCISE
Ques. 1: Which photosynthetic pigment moves farthest and why?
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Carotene moves the farthest because it is the most nonpolar of the pigments and it is attracted more strongly to the acetone-ligroin mixture (mobile phase) than to the paper. This stronger, nonbonded interaction with the mobile phase indicates that carotene is the most nonpolar pigment found in spinach chloroplasts.
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