Experiment to Investigate Diffusion in a Jelly
Hypothesis :
Required Materials:
Results:
Conclusion:
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Answers
Answer:
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Explanation:
Agar Cell Diffusion
Use cubes of agar to investigate how size impacts diffusion.
Tools and Materials:
- Agar-agar powder
- Digital scale
- Graduated cylinder
- Water
- Whisk or fork
- Microwaveable bowl or container at least 500ml in volume
- Microwave (not shown)
- Hot pad or oven mitt
- Heat-safe surface
- pH indicator, such as bromothymol blue or phenolphthalein
- Ammonia
- Small glass baking pan or cube-shaped silicone ice-cube molds
- Clear plastic metric ruler
- Sharp knife
- Clear container for immersing agar cubes
- Vinegar
- Calculator
- Pencil and notepaper
- Spoon
- White paper or plate
- Timers
Assembly:
1. Measure out 1.6 g of agar-agar and 200 ml water. Mix them together with a whisk or fork in a large microwaveable bowl.
2. Heat the solution in the microwave on high for 30 seconds. Remove to a heat-safe surface using a hot pad or oven mitts, stir, and return to the microwave for 30 seconds. Repeat this process until the mixture boils. (Keep your eye on it, as it can boil over very easily!) When done, remove the container and set it on a trivet or other heat-safe surface.
3. Choose ONE pH indicator to work with (either bromothymol blue or phenolphthalein) and add a few
drops of it to the agar solution. If you’re using bromothymol blue, add enough indicator so that the mixture turns blue. If it has a greenish hue, add ammonia one drop at a time until it is blue. If you're using
phenolphthalein, add enough indicator so that the mixture turns pale pink. Add ammonia drop by drop until the mixture turns (and remains) bright pink.
4. Carefully pour the agar solution into silicone ice-cube molds or a small glass baking pan. Make sure the agar block(s) will be at least 3 cm deep when they solidify. If you don’t have enough solution, make
more using the ratio of 0.8 g agar powder to 100 ml water.
5. Let the agar cool until it solidifies (an hour is usually sufficient). Remove the agar blocks from the molds or cut in the pan with a sharp knife to obtain two sets of cubes of three sizes: 1 x 1 x 1 cm, 2 x 2 x 2 cm, and 3 x 3 x 3 cm. If you're using bromothymol blue, you should have two sets of blue cubes. If you're using phenolphthalein, you should have two sets of pink cubes. The sets need to match in order to compare the color change later on in the Snack.