Experimental method to calculate volumetric mass transfer coefficient in bio reactors
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The volumetric mass transfer coefficient (kLa) is often used in order to compare the efficiency of bioreactors and as an important scale-up factor. In bioreactors, a number of methods are available for estimation the overall volumetric oxygen mass transfer coefficient: the dynamic method, the steady-state method based on a preliminary determination of the oxygen uptake rate, the gaseous oxygen balance, the carbon dioxide balance. Each method provides a different estimation of the value of kLa. We examined the sensitivity of the various methods and compare the kLa values obtained in order to select the most suitable method as a function of the type of bioreactor used. In this work we applied dynamic gassing-out method for measuring the volumetric mass transfer coefficient kLa in three type gas-liquid reactors (stirred tanks, bubble columns and airlift). Тhe aim of this work is on the basis of our experimental data to obtain several correlations for evaluation of kLa. Keywords: volumetric oxygen transfer coefficient, bubble columns, CSTR, airlift reactors.
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