Experimentally Show That Carbon Dioxide Is needed for photosynthesis.(5 marks)
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Insert a part of the leaf of a destarched plant into a conical flask containing potassium hydroxide. Potassium hydroxide solution absorbs the carbon dioxide gas from the air present in the glass bottle. Leave the plant sunlight. After few hours, perform a starch test to this and another leaf of the same plant
To prove that CO2 is necessary for photosynthesis the following experiment can be formed:
1. A potted plant is kept in dark for three days so that the leaves become free from starch.
2. A healthy leaf is selected for experiment.
3. a wide mouth bottle is taken and a little KOH (it absorbs CO2) is added to it.
4.Half part of the leaf is kept in the bottle with the help of split cork.
5. The apparatus in kept in bright sunlight for few hours.
6. The leaf is plucked and boil in 70% alcohol. It is stained with iodine solution.
Observation : It was observed that the portion of leaf which was out of the bottle turned blue showing the presence of starch. The portion on leaf which was enclosed within the bottle will not show the presence of starch
Conclusion : This shows that CO2 is essential for photosynthesis, because the portion inside the bottle did not get the CO2 as it was absorbed by KOH solution. Thus CO2 is necessary for photosynthesis.
