Experiments show that h2o is essential for photosynthesis
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Stable isotopes of carbon and hydrogen are useful indicators of the photosynthetic pathway.
Use a hydrogen isotope labelled water to see that the water is split in the photolysis reaction. Then track the hydrogen ions to see where they end up in the plants.
2 (H2O) --- photolysis reaction --> O2 + ATP + NADPH (carrier molecule will transfer H isotope to calvin cycle if water provides the H to build glucose)
NADPH transfers to the Calvin cycle step of photosynthesis and provides the hydrogen to build the CO2 into an organic molecule.
Photosynthesis
CO2 + H2O → → O2 + CH2O
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