Question

Explain
1. PCR
2. Chitinase​

Answer 1

Explanation:

Chitinase gene has been widely studied as one of the pest control agents because of its characteristic of degrading chitin. Chitinase is found in insects, nematodes, fungi, some algae and yeast. Beauveria bassiana is an insect pathogenic fungus successfully used as an insect pest control agent worldwide.

Polymerase chain reaction, or PCR, is a technique to make many copies of a specific DNA region in vitro (in a test tube rather than an organism). ... In PCR, the reaction is repeatedly cycled through a series of temperature changes, which allow many copies of the target region to be produced.

Answer 2

Explanation:

1.Polymerase chain reaction, or PCR, is a technique to make many copies of a specific DNA region in vitro (in a test tube rather than an organism). PCR relies on a thermostable DNA polymerase, Taq polymerase, and requires DNA primers designed specifically for the DNA region of interest.

2.Chitinase − Chitinase is a class of enzymes used for the degradation of chitin, which forms a major component of the fungal cell wall. Therefore, to isolate the DNA enclosed within the cell membrane of the fungus, enzyme chitinase is used to break the cell for releasing its genetic material.

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