Question

explain any two method for introduction of recombinant DNA technology? please give ANS......

Answer 1

Method # 1. Phage Introduction or Transfection:

Phage introduction is the process of transfection which is equivalent to transformation excepting that a phage is used instead of bacterial plasmid. In vitro packaging, a vector uses lambda or M 13 phages to produce phage plaques which contain recombinant DNA. The recombinant DNA can be identified using various selection methods. For the first time bacteriophage was used to transfer the foreign DNA into E. coli cells.

If the vector is bacteriophage, its replication in bacterial host would result in phage particles, each carrying an identical copy of target gene. When phage vectors are used, a population of cells is infected with the viruses and virus replication proceeds spontaneously.

Eventually, the phage DNA containing the target gene is inserted into the bacterial chromosome where it will replicate as though it was a part of normal chromosome. Thus these vectors along with target genes are introduced into a bacterial host. This is usually achieved with the enzyme DNA ligase. It is important for ligation that vector DNA and the target DNA have been cut by the same restriction endonuclease.

Recombinant DNA Formation: Method # 2. Non-Bacterial Transformation:

This process is similar to transformation. The only different between the two is that non-bacterial transformation does not use bacteria such as E. coli as the host. In microinjection the DNA fragment is injected directly into the nucleus of the cell be| transformed.

In biolistics, the host cells are bombarded with high velocity micro-projectile gun such as particles of gold or tungsten that have been coated with DNA

Answer 2

Method of recombinant DNA technology

Explanation:

1. Transformation: The limitation protein which causes a break in remote DNA additionally causes an amazing cut in the vector DNA at a particular cleavage site. The durable parts of the bargains have the arrangements of nucleotides corresponding to the strong parts of the bargains. This can be shown utilizing a case of compound Eco RI, comparable clingy closes having a similar base succession in single-stranded closures will be created.

2. Phage Introduction or Transfection:  Phage presentation is the procedure of transfection which is comparable to change aside from that a phage is utilized rather than a bacterial plasmid. In vitro bundling, a vector utilizes lambda or M 13 phages to deliver phage plaques that contain recombinant DNA. The recombinant DNA can be distinguished utilizing different determination techniques. Just because bacteriophage was utilized to move the remote DNA into E. coli cells.