Question

Explain Benedict's test.

Answer 1

The benedict's solution is a chemical reagent formed as a mixture of sodium carbonate,try sodium citrate dihydrate and copper (II) sulphate pentahydrate. It is used to test the presence of reducing sugar group.

Actually, it has a blue color due to copper sulphate solution and, it gives either red or green or yellow precipitate if added to a reducing sugar compound solution.

Answer 2

Answer:

Benedict's test is the test done to detect the presence of sugar in urine sample using Benedict's solution.

The materials required for the experiment :

• Test tube
• Measuring flask
• Test tube holder
• Burner
• Benedict's solution
• Dropper

Procedure :

1. Take 2 ml urine sample in a measuring flask.
2. Transfer it in a test tube.
3. Add 2 ml of Benedict's solution to the measuring flask.
4. Transfer it to the test tube.
5. Heat the test tube for 2 minutes.
6. Stir the test tube while burning.

Observation :

• If the colour is blue, that is unchanged, there is no sugar in the urine sample.
• If the colour is green, there is a presence of sugar in traces.
• If the colour is yellow, there is presence of sugar.
• If the colour is orange or brick red, the quantity of the sugar in urine sample is large to maximum.