Question

Explain Denaturation and renaturation

Answer 1

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✔Denaturation causes a marked decrease in viscosity. If melted DNA is cooled it is possible to reassociate the separated strands, a process known as renaturation. However, a stable double-stranded molecule may be formed only if the complementary strands collide in such a way that their bases are paired precisely.

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Answer 2

Separation of two strands of DNA from each other due to breakage of H-bonds when it is exposed to high temperature acid or alkali is called denaturation on melting. Reassociation of separated DNA by H-bonds formation is called renaturation and annealing. DNA with more A T has low melting areas and denatured more easily DNA with more than G C has high melting areas.