Explain determination method of n terminal amino acid
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Determining which amino acid forms the N-terminus of a peptide chain is useful for two reasons: to aid the ordering of individual peptide fragments' sequences into a whole chain, and because the first round of Edman degradation is often contaminated by impurities and therefore does not give an accurate determination of it...
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A generalised method for N-terminal amino acid analysis follows: React the peptide with a reagent that will selectively label the terminal amino acid. Hydrolyse the protein. Determine the amino acid by chromatography and comparison with standards.
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