Explain different types of sensors with their functions that were used in the chromatography of GFP.
Answers
Answer:
Green fluorescent protein (GFP) is a 27 kD protein consisting of 238 amino acid residues [1]. GFP was first identified in the aquatic jellyfish Aequorea victoriaby Osamu Shimomura et al. in 1961 while studying aequorin, a Ca2+-activated photoprotein.Aequorin and GFP are localized in the light organs of A. victoriaand GFP was accidentally discovered when the energy of the blue light emitted by aequorin excited GFP to emit green light.Unlike most fluorescent proteins which contain chromophores distinct from the amino acid sequence of the protein, the chromophore of GFP is internally generated by a reaction involving three amino acid residues [2]. This unique property allows GFP to be easily cloned into numerous biological systems, both prokaryotic and eukaryotic, which has paved the way for its utilisation in a variety of biological applications, most notably in biosensing.
Answer:
Biologists use GFP to study cells in embryos and fetuses during developmental processes. Biologists use GFP as a marker protein. GFP can attach to and mark another protein with fluorescence, enabling scientists to see the presence of the particular protein in an organic structure.