Question

Explain Dilutions with eg​

Answer 1

DILUTION

Dilutions: Explanations and Examples of Common Methods

There are many ways of expressing concentrations and dilution. The following is a brief explanation of some ways of calculating dilutions that are common in biological science and often used at Quansys Biosciences.

Using C1V1 = C2V2

To make a fixed amount of a dilute solution from a stock solution, you can use the formula: C1V1 = C2V2 where:

V1 = Volume of stock solution needed to make the new solution

C1 = Concentration of stock solution

V2 = Final volume of new solution

C2 = Final concentration of new solution

Example: Make 5 mL of a 0.25 M solution from a 1 M solution

Formula: C1V1 = C2V2

Plug values in: (V1)(1 M) = (5 mL)(0.25 M)

Rearrange: V1 = [(5 mL)(0.25 M)] / (1 M)V1 = 1.25 mL

Answer: Place 1.25 mL of the 1 M solution into V1-V2 = 5 mL – 1.25 mL = 3.75 mL of diluent