explain how does an antibiotic resistance gene in a cloning vector (plasmid pBR322) help in selecting the recombinant from the non-recombinant
Answers
Answer:
Antibiotic resistance genes help in selecting recombinants from non-recombinants by a method called insertional inactivation where a recombinant DNA is inserted within the coding sequence of an enzyme β-galactosidase in the presence of a chromogenic substrate which results into inactivation of the enzyme.
Answer: The pBR322 is a vector that is used for the recombination of genetic material to produce hybrids with desired traits and the importance of antibiotic resistance or lac Z gene is that it helps differentiate the transformed cells from non transformants.
Explanation: For eg the E. coli bacteria is suceptible to antibiotics and to grow in the media with antibiotics it needs to attain resistance and this is done by vector pBR322 which differentiates as if any antibiotic sensitive bacteria is transformed with non recombinated plasmid the bacteria would produce blue color and lac Z gene insertion with new DNA decides or differentiates as if lac Z is inactive by insertion the colonies produce white color and the DNA is recombinant otherwise if lac Z gene is active that means new DNA is not inserted and colony will be blue.
#SPJ3