Question

Explain how recombinant DNA is inserted into the host cell ?

Answer 1

Recombinant DNA technology, joining together of DNA molecules from two different species that are inserted into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture, and industry.

Answer 2

Dear Student,

◆ Insertion of desired gene in the plasmid -

• First plasmid DNA is cut at specific sites by restriction endonuclease
• This will produce sticky ends in the plasmid
• Now the desired gene is inserted into the plasmid

◆ Insertion of rDNA into the host cell -

• Plasmids are inserted into host cell by transformation

◆ Steps in rDNA technology -

• Isolate vector plasmid and human DNA
• Insert human DNA into plasmid
• Put plasmids into host bacteria by transformation
• Multiplication of rDNA in bioreactor to produce many copies
• Identify clone carrying DNA of interest

Thanks dear. Hope this is helpful to you ..