Question

Explain how to find whether an E. coli bacterium has been transformed or not when a recombinant DNA bearing ampicillin resistant gene is transferred into it. (b) What does the ampicillin resistant gene act as in the above case?

Answer 1

Heyaa☺

_____________________________

➡To find whether an E.coil bacterium has been transformed or not when a recombinant DNA bearing amplicillin resistant gene is transferred into it is the recombinant plasmid will lose resistance due to insertion of DNA which is if foreign and it can still be selected out from non recombinant ones by plating the transformanrs on ampicillin containing medium.Non recombinant will grow on the medium containing both antibotics.

➡The ampicillin resistance gene act as selectable marker as it helps in selecting the transformats and medium.

________________________

Hope you understand..✌