Explain in detail principle of HPLC. Give an account of columns, pumps and detectors used in HPLC.
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HPLC works on the principle that some molecules take longer than others to pass through a chromatography column. This depends on the affinity of the molecule with the mobile phase (liquid or gas) and the stationary phase (solid or liquid).
High-performance liquid chromatography or high-pressure liquid chromatography (HPLC) is a chromatographic method that is used to separate a mixture of compounds in analytical chemistry and biochemistry so as to identify, quantify or purify the individual components of the mixture.
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