Question

Explain in detail the process of transcription in eukaryotes.

Answer 1

The initiation of gene transcription in eukaryotes occurs in specific steps. First, an RNA polymerase along with general transcription factors binds to the promoter region of the gene to form a closed complex called the preinitiation complex.

Answer 2

Transcription In Eukaryotes

The primary transcript hn RNA ( Heterogenous Nuclear RNA ) contains two types of segments ;

• Non coding introns

• Coding exons.

The primary eukaryotic mRNA transcript is non functional.

It undergoes three types of processing;

1) SPLICING : Where the introns are removed and exons are joined in a definite manner.

2) CAPPING: An unusual nucleotide methyl guanosine triphosphate is added to the 5’ end of the hnRNA.

3) TAILING: Adenylate residues are added ( polyadenylation ) at 3’end of hnRNA.

The fully processed hnRNA or mRNA is transferred outside the nucleus.