Explain in sequence the process of
amplification of gene of interest using
polymerase chain reaction.
Answers
Answer:
Answer:Polymerase chain reaction (PCR) is the process in which the amplification of the gene of interest is carried out with two sets of primers and a thermostable DNA polymerase enzyme Taq polymerase. The process involves the following steps:
Answer:Polymerase chain reaction (PCR) is the process in which the amplification of the gene of interest is carried out with two sets of primers and a thermostable DNA polymerase enzyme Taq polymerase. The process involves the following steps: Denaturation: The double-stranded DNA is heated up to 94° C which causes the hydrogen bonds to break and the two strands get separated.
Answer:Polymerase chain reaction (PCR) is the process in which the amplification of the gene of interest is carried out with two sets of primers and a thermostable DNA polymerase enzyme Taq polymerase. The process involves the following steps: Denaturation: The double-stranded DNA is heated up to 94° C which causes the hydrogen bonds to break and the two strands get separated.Annealing: The two sets of primers are added which bind to the appropriate complementary segment of DNA strand at 54°C.
Answer:Polymerase chain reaction (PCR) is the process in which the amplification of the gene of interest is carried out with two sets of primers and a thermostable DNA polymerase enzyme Taq polymerase. The process involves the following steps: Denaturation: The double-stranded DNA is heated up to 94° C which causes the hydrogen bonds to break and the two strands get separated.Annealing: The two sets of primers are added which bind to the appropriate complementary segment of DNA strand at 54°C.Extension: The Taq polymerase enzyme polymerizes the nucleotide chain using the nucleotides provided in the medium and by using the template strand at 72°C.