Question

Explain native PAGE and give its applications.

Answer 1

Native" or "non-denaturing" gel electrophoresis is run in the absence of SDS. While in SDS-PAGE the electrophoretic mobility of proteins depends primarily on their molecular mass, in native PAGE the mobility depends on both the protein's charge and its hydrodynamic size.

Native gel electrophoresis is mainly used to check the purity of the protein; to find the number of protein present in the sample. SDS-PAGE is used to find the molecular weight of the protein. Native or non denaturing gel electrophoresis is run in the absence of SDS.

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