Question

explain paper chromatography?​

Answer 1

Answer:

Chromatography technique that uses paper sheets or strips as the adsorbent being the stationary phase through which a solution is made to pass is called paper chromatography. It is an inexpensive method of separating dissolved chemical substances by their different migration rates across the sheets of paper.

Explanation:

The principle involved can be partition chromatography or adsorption chromatography. Partition chromatography because the substances are partitioned or distributed between liquid phases. The two phases are water held in pores of the filter paper and the other phase is a mobile phase which passes through the paper. When the mobile phase moves, the separation of the mixture takes place. The compounds in the mixture separate themselves based on the differences in their affinity towards stationary and mobile phase solvents under the capillary action of pores in the paper. Absorption chromatography between solid and liquid phases, wherein the solid surface of the paper is the stationary phase and the liquid phase is the mobile phase.

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Answer 2

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$\Large\bold\blue{Partition\:chromatography}$

◇ Paper chromatography (PC) is an example of Partition Chromatography.

◇The same procedure is followed as in thin layer chromatography except that a strip of paper acts as an adsorbent.

◇This method involves continues differential portioning of components of a mixture between stationary and mobile phase.

◇In paper chromatography, a special quality paper known as chromatography paper is used. This paper act as a stationary phase.

◇ A strip of chromatographic paper spotted at the base with the solution of the mixture is suspended in a suitable solvent which act as the mobile phase.

◇The solvent rises up and flows over the spot. The paper selectively retains different components according to their different partition in the two phases where a chromatogram is developed.

◇The spots of the separated colored compounds are visible at different heights from the position of initial spots on the chromatogram.

◇The spots of the separated colorless compounds may be observed either under ultra violent light or by the use of an appropriate spray reagent.

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