Explain paper chromatography...
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Answer:
Paper chromatography is an analytical method used to separate colored chemicals or substances.[1] It is primarily used as a teaching tool, having been replaced by other chromatography methods, such as thin-layer chromatography. A paper chromatography variant, two-dimensional chromatography involves using two solvents and rotating the paper 90° in between. This is useful for separating complex mixtures of compounds having similar polarity, for example, amino acids. The setup has three components. The mobile phase is a solution that travels up the stationary phase, due to capillary action. The mobile phase is generally mixture of non-polar organic solvent, while the stationary phase is polar inorganic solvent water. Here paper is used to support the stationary phase, water. Polar water molecules are held inside the void space of cellulose network of the host paper. Difference between TLC and paper chromatography is that stationary phase in TLC is a layer of adsorbent (usually silica gel, or aluminium oxide), and stationary phase in paper chromatography is water.
Explanation:
Answer:
In paper chromatography, chromatography paper is used which contains water trapped in it which is the stationary phase. On the base of the paper, a solution (containing the mixture to be separated) is spotted. The paper strip is suspended in suitable solvent which is mobile phase. The solvent rises to the top of the paper by capillary action. During this, it flows
over the spot. The components are selectively retained on the paper. The spots of different components travel with the mobile phase to different heights. The developed paper is called chromatogram