explain principal of partition chromatography
Answers
The principle of partition chromatography is that the separation of components of given sample occurs due to partition of components between two liquid phases. In this process, the stationary phase is coated with a liquid surface. The solid surface must be immiscible in the mobile phase.
Answer:
Medical Definition of partition chromatography
: a process for the separation of mixtures in columns or on filter paper based on partition of a solute between two solvents one of which is immobilized by the substance in the column or by the paper.
Partition Chromatography Definition
Partition Chromatography technique is defined as the separation of components between two liquid phases viz original solvent and the film of solvent used in column. This separation theory was introduced in the year 1940s which was published by Richard Laurence Millington Synge and Archer Martin. It is also known as Liquid-liquid chromatography (LLC). Or if gas is the mobile phase it is called Gas-liquid chromatography (GLC)
Partition Chromatography Principle
The separation of the components from the sample mixture is carried out by the process of partition of the components between 2 phases. Both the phases are in liquid form. In this process the immiscible solid surface coated with the liquid surface on the stationary phase is in the mobile phase. The liquid surface is immobilized by stationary phase which results in making it stationary phase. The mobile phase moves from the stationary phase and components get separated. The separation depends on different partition coefficient.
Explanation:
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