Question

explain principle of chromatography​

Answer 1

Answer:

Chromatography is based on the principle where molecules in mixture applied onto the surface or into the solid, and fluid stationary phase (stable phase) is separating from each other while moving with the aid of a mobile phase.

Answer 2

Answer:

• Paper chromatography is a type of partition chromatography, which is based on continuous differential partitioning of components of a mixture between a stationary phase and a mobile phase
• A special quality paper (called chromatography paper) is used as the adsorbent column or a statis phase. The paper contains water trapped in it. The paper is suspended in a suitable solvent which is the mobile phase.
• The solvent rises up the paper and flows over the spot due to capillary action.
• The paper selectively retains different components according to their differing partition in two phases.
• The paper strip so developed is called chromatogram.