Explain r DNA technology ?????????
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Answer:
Recombinant DNA Technology is defined by the Encyclopedia Britannica as “the joining together of DNA molecules from different organisms and inserting it into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture and industry.
➡Recombinant DNA is the term applied to chimeric DNA molecules that are constructed in vitro, then propagated in a host cell or organism.
➡The basic recombinant DNA consists of a vector and an insert The vector is a replicon capable of replicating in the cells of choice
. ➡It is endowed with a functional replication origin, usually carries a selectable marker, and typically has been engineered to accommodate inserts conveniently.
➡Vectors are based on naturally occurring replicons, such as bacterial plasmids, viruses, or cellular chromosomes.
➡inserts can be of any sort – long or short segments of DNA, from natural or synthetic sources.
➡The resulting recombinant DNAs are often referred to as clones, which is shorthand for chimeric DNAs that are isolated in cellular or viral clones; and the process of producing these recombinants is frequently called DNA cloning or gene cloning.
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