Explain : Tha mechanism and importance of PCR
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The polymerase chain reaction (PCR) was originally developed in 1983 by the American biochemist Kary Mullis. ... Using PCR it is possible to generate thousands to millions of copies of a particular section of DNA from a very small amount of DNA. PCR is a common tool used in medical and biological research labs.
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Mechanism-
To amplify a segment of DNA using PCR, the sample is first heated so the DNA denatures, or separates into two pieces of single-stranded DNA. Next, an enzyme called "Taq polymerase" synthesizes - builds - two new strands of DNA, using the original strands as templates.
Importance-
The polymerase chain reaction (PCR) is used to make millions of copies of a target piece of DNA. It is an indispensable tool in modern molecular biology and has transformed scientific research and diagnostic medicine.
To amplify a segment of DNA using PCR, the sample is first heated so the DNA denatures, or separates into two pieces of single-stranded DNA. Next, an enzyme called "Taq polymerase" synthesizes - builds - two new strands of DNA, using the original strands as templates.
Importance-
The polymerase chain reaction (PCR) is used to make millions of copies of a target piece of DNA. It is an indispensable tool in modern molecular biology and has transformed scientific research and diagnostic medicine.
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