Explain the activity. peel of the leaf-Dermal tissue.
Answers
Explanation:
Objective:
To prepare a temporary mount of a leaf peel to show stomata.
Apparatus and Materials Required:
A potted Tradescantia or Bryophyllum plant, forceps, needles, watch glasses, glass slides, a dropper, coverslips, a brush, blotting paper, safranin, glycerine and a compound microscope.
Procedure:
1. Remove a healthy leaf from the potted plant.
2. Remove a part of the peel from the lower surface of the leaf. You can do this by folding the leaf over and gently pulling the peel apart using forceps. Keeps the peel in a watch glass containing water.
3. Put a few drops of safranin stain in a watch glass.
4. After 2-3 minutes take out the peel and place it on a clean glass slide.
5. Put a drop of glycerin over the peel and place a clean coverslip gently over it with the help of a needle.
6. Remove the excess stain and glycerin with the help of blotting paper.
7. Observe the slide under the low-power and high-power magnifications of the compound microscope
Observations:
1. The epidermal cells are visible. These are irregular in outline and have no intercellular spaces.
2. Many small pores (stomata) are seen scattered among the epidermal cells.
3. Each pore is guarded by two bean-shaped guard cells, each containing chloroplasts and a nucleus.
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