Explain the application of rDNA technology to
produce insulin.
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This process is called recombinant technology. The bacterial DNA now contains the human insulin gene and is inserted into a bacteria. When the bacterial cells reproduce, the human insulin gene is also reproduced in the cells and produces protein by transcription and translation process.
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ʜᴜᴍᴀɴ ɪɴsᴜʟɪɴ ɪs sʏɴᴛʜᴇsɪsᴇᴅ ᴀs ᴀ ᴘʀᴏ-ʜᴏʀᴍᴏɴᴇ. ᴛʜᴇ ᴘʀᴏ-ʜᴏʀᴍᴏɴᴇ ᴄᴏɴᴛᴀɪɴs ᴀɴ ᴇxᴛʀᴀ ᴄ-ᴘᴇᴘᴛɪᴅᴇ.ᴛʜᴇ ᴄ-ᴘᴇᴘᴛɪᴅᴇ ɪs ɴᴏᴛ ᴘʀᴇsᴇɴᴛ ɪɴ ᴍᴀᴛᴜʀᴇ ɪɴsᴜʟɪɴ ᴀɴᴅ ɪs ʀᴇᴍᴏᴠᴇᴅ ᴅᴜʀɪɴɢ ᴍᴀᴛᴜʀᴀᴛɪᴏɴ. ᴇʟɪ-ʟɪʟʏᴀɴ ᴀᴍᴇʀɪᴄᴀɴ ᴄᴏᴍᴘᴀɴʏ ᴘʀᴇᴘᴀʀᴇᴅ ᴛᴡᴏ ᴅɴᴀ sᴇǫᴜᴇɴᴄᴇs, ᴄᴏʀʀᴇsᴘᴏɴᴅɪɴɢ ᴛᴏ ᴀ ᴀɴᴅ ʙ ᴄʜᴀɪɴs ᴏғ ʜᴜᴍᴀɴ ɪɴsᴜʟɪɴ ᴀɴᴅ ɪɴᴛʀᴏᴅᴜᴄᴇᴅ ᴛʜᴇᴍ ɪɴ ᴘʟᴀsᴍɪᴅs ᴏғ ᴇ. ᴄᴏʟɪ ᴛᴏ ᴘʀᴏᴅᴜᴄᴇ ɪɴsᴜʟɪɴ ᴄʜᴀɪɴs. ᴄʜᴀɪɴ ᴀ ᴀɴᴅ ʙ ᴡᴇʀᴇ ᴘʀᴏᴅᴜᴄᴇᴅ sᴇᴘᴀʀᴀᴛᴇʟʏ, ᴇxᴛʀᴀᴄᴛᴇᴅ ᴀɴᴅ ᴄᴏᴍʙɪɴᴇᴅ ʙʏ ᴄʀᴇᴀᴛɪɴɢ ᴅɪsᴜʟᴘʜɪᴅᴇ ʙᴏɴᴅs.
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