Question

Explain the experimental process of observing a Nuclear
in the check cell.​

Answer 1

Answer:

Explanation:

Materials needed:

Sterile cotton swab

Clean, sterile microscope slides

Microscope cover slips

Methylene Blue solution (0.5% to 1%)

Dropper

Blotting paper/Tissue paper

Microscope

Procedure

before starting, it's always important to ensure that the working surface is clean and that you are wearing a pair of clean gloves to avoid contamination.

Cheek cells can be easily obtained by gently scraping the inside of the mouth using a clean, sterile cotton swab.

Once the cells have been obtained, the following procedure is used for cheek cell wet mount preparation:

place a drop of physiological saline on a clean microscopic slide (central part of the slide)

smear the cotton swab on to the center (part containing the saline drop) of the clean slide for about 4 seconds to get the cells on to the center of the slide

add a drop of methylene blue solution on to the smear and gently place a cover slip on top (to cover the stain and the cells)

any excess solution can be removed by touching one side of the slide with a paper towel or blotting paper.

place the slide on the microscope for observation using 4 x or 10 x objective to find the cells

once the cells have been found, they can then be viewed at higher magnificatio

Observation

Large irregularly shaped cells with distinct cell membranes.

A distinct nucleus at the central part of each individual cell (dark blue in color).

A lightly stained cytoplasm in each cell.

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