Question

Explain the features required to facilitate cloning into a vector. What do you mean by cloning sites. explain briefly.

Answer 1

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Answer : Plasmid cloning vectors contain different components that help in successful cloning of gene of interest.

These components are: Cloning sites: Cloning sites refer to the recognition sites for restriction enzymes (such as EcoRI, Hind III, PvuI , BamHI, etc.) present on cloning vector.


Also it helps in identifying and eliminating non transformants and selectively permitting the growth of the transformants.Transformation is a procedure through which a piece of DNA is introduced in a host bacterium.
The genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are useful selectable markers for E. coli as the normal E. coli cells do not carry resistance against any of these antibiotics.