Question

explain the hershey-chase experiment. ​

Answer 1

• In 1952 Alfred Hershey and Martha Chase made two preparations of T2 phage.

• In one preparation the proteins were labelled with S35.

• In the second preparation the DNA was labelled with P32.

• They were used separately to infect E.coli.

• Just after infection the E.coli cells were gently agitated in a blender, to separate the phage particles from the bacteria.

• Then the culture was centrifuged, the heavier bacterial cells formed a pellet at the bottom.

• The lighter viral components outside the bacterial cells remained in the supernatant.