Explain The Mechanism Of Elisa. What Are Its Uses?
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ELISA is the basic assay technique, known as enzyme-linked immunosorbent assay (also referred to as EIA: Enzyme Immunoassay) that is carried out to detect and measure antibodies, hormones, peptides and proteins in the blood. Antibodies are blood proteins produced in response to a specific antigen.
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Performing an ELISA involves at least one antibody with specificity for a particular antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. ...
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