Question

Explain the method to increase the competency of the bacterial cell membrane to take up recombinant DNA?

Answer 1

(a) Chemical method In this method, the cell is treated with a specific concentration of a divalent cation such as calcium to increase pore size in cell wall. them briefly at 42°C and then putting it back on ice. This is called heat shock treatment. This enables the bacteria to take up the recombinant DNA.