explain the method to increase the competency of the bacterial cell membrane to take recombinant DNA
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The Recombinant DNA can be forced into the bacterial cell treated with divalent cations and incubating it with recombinant DNA on ice. This is to be followed by placing it briefly at 42°C (heat shock), and then putting it back on ice. This process would enable the bacteria to take up the recombinant DNA.
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