explain the principle of paper chromatography
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- Paper chromatography is a type of partition chromatography, which is based on continuous differential partitioning of components of a mixture between a stationary phase and a mobile phase
- A special quality paper (called chromatography paper) is used as the adsorbent column or a statis phase. The paper contains water trapped in it. The paper is suspended in a suitable solvent which is the mobile phase.
- The solvent rises up the paper and flows over the spot due to capillary action.
- The paper selectively retains different components according to their differing partition in two phases.
- The paper strip so developed is called chromatogram.
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Principle of paper chromatography:
The principle involved is partition chromatography wherein the substances are distributed or partitioned between liquid phases. One phase is the water, which is held in the pores of the filter paper used; and other is the mobile phase which moves over the paper.
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